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This article describes experiments carried out to evaluate the impact of physical variables of agitated tank fermentation on the process performance and product quality profile of therapeutic mAbs The aim of these experiments was to develop a consistent fermentation manufacturing process 5 for a therapeutic biosimilar mAb that would maintain
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Further DetailsMicrobial cultures require highly developed specialized capabilities such as increased demand for aeration and agitation The high growth rates result in rapidly changing conditions which require fast response times accurate sensors and reliable controls for pH DO temperature in order to control the culture conditions
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Further DetailsMar 01 2020 · 22 Chemicals and equipment All experiments used analytical grade reagents purchased from Sigma Aldrich Saint Louis MO The 1000 mgL aqueous AsV stock solution was prepared by dissolving HAsNa 2 O 4 ·7H 2 O in deionized water Solution pH was adjusted using either 01 M HCl or 01 M NaOH Hanna HI 2211 pHORP Meter
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